NucleoBond Xtra Midi Plus (50) 740412.50 Application: Transfection-grade plasmid DNA isolation Target: Plasmid DNA Technology: Anion exchange chromatography Brand: NucleoBond Format: Midi prep Package unit: 50 Preps. Handling: Gravity flow Lysate clarification: Column filter Sample material: Bacteria, E. coli Sample amount: < 200 mL (high copy), < 400 mL (low copy) Vector size : 2−50 kbp Typical yield: 500 μg Theoretical binding capacity: 800 μg Typical purity A260/A280: 1.8−1.95 Endotoxin level: 1−10 EU/μg DNA Isopropanol precipitation: NucleoBond Finalizer Preparation time: 30 min/prep Typical downstream application: Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells. Storage temperature: 18−25 °C Shelf life (from production): 39 Months Producer: Macherey-Nagel.