Enzymatic digest of casein.........................1.00 Yeast extract.............................................. 2.00 Sodium chloride..........................................5.00 Disodium phosphate..................................2.70 Monosodium phosphate............................. 2.20 Tryptophan................................................. 1.00 Sodium pyruvate........................................ 1.00 Tergitol®7.................................................... 0.15 Sorbitol....................................................... 1.00 6-Chloro-3-indoxylß- D-galactopyranoside............................... 0.20 5-Bromo-4-chloro-3-indoxyl-ß-D-glucuronic acid........................0.10 IPTG........................................................... 0.10 Agar..........................................................13.00 Final pH 6,8 ±0,2 at 25ºC mai putin