kit Giemsa pentru Helicobacter pylori, 15x50ml, produs original Bio-Optica/Italia. Application To demonstrate Helicobacter pylori bacterium on gastric biopsy sections. The qualitative and quantitative composition of staining mixture and the careful differentiation allow to identify bacteria on tidy ground. Warning The kit allows to prepare 15 assays using Coplin Jars of 50 ml. Each assay stains from 1 to 5 sections. Reagent can be introduced into the Coplin Jar and then recovere Method Deparaffinize sections and bring to distilled water. Preparation of buffer solution. Take out from bottle B 5 ml of solution and diluting 1:10: Utilize obtained solution for the preparation of Giemsa working-solution. Preparation of Giemsa working-solution. Take out 10 ml of reagent A and reach the volume of 40 ml with buffer solution previously prepared. Put the solution in a Coplin Jar and immerse sections for 30 minutes. Drain the slide without washing and put the section for 15 seconds in reagent C. Repeat step 5 with reagent D and E. Clear in xylene and mount. Results Helicobacter pylori Nuclei Cytoplasm blue, in form of seagull's wing blue pink Reagents A) Giemsa solution modified 150 ml B) Acetate buffer solution 150 ml C) Differentiating reagent 150 ml D) Dehydrating reagent 150 ml E) Dehydrating reagent 150 ml kit 75 teste Podusul (Dispozitivul medical) dispune de certificare pentru utilizare in Diagnosticul In-Vitro (IVD) mandatoriu pentru produsele utilizate in