Formula* (in g/L) Peptone 5,000 Dextrose 10,000 Monopotassium phosphate 1,000 Magnesium sulphate. 7H2O 0,500 Dichloran 0,002 Chloramphenicol 0,100 Agar 15,000 Final pH at 25ºC 5,6 ± 0,2 Tryptone.................................................10,00 Lithium chloride......................................15,00 Proteose peptone...................................10,00 Sodium chloride....................................... 5,00 Yeast extract............................................ 3,00 Starch.......................................................1,00 Esculin..................................................... 1,00 Ferric ammonium citrate.......................... 0,50 Agar....................................................... 13,00 Final pH 7,2 ±0,2 at 25ºC Tryptose..............................20,0 Yeast extract....................5,0 Dextrose............................ 2,0 Potassium phosphate........................ 4,0 Sodium azide..................0,4 Agar...................................12,0 Final pH 7,0 ± 0,2 flacon de 500g Specification Selective and differential solid medium for the detection and enumeration of ?-glucuronidase-positive Escherichia coli according ISO standards 16649-1 & -2 Formula (in g/L) Tryptone 20.000 Bile Salts #3 1.500 X-?-D-Glucuronide 0.075 Agar 15.000 Final pH 7.0 (?0.2)