CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay - Option2 Custom oligo mix #of oligos per mix 12 #of reactions 20,000 probes will be HPLC purified primers will be synthesized and standard desalted and normalized The oligos will be mixed together and resuspended in IDTE buffer pH 7.5 Option 2: Assay1: Sequence name Sequence yield (in nmol) InfA /56-FAM/TTT GTG TTC/ZEN/ ACG CTC ACC GTG CC/3IABkFQ/ 51.2 yield (in nmol) InfB /5YakYel/AGA AGA TGG/ZEN/ AGA AGG CAA AGC AGA ACT AGC/3IABkFQ/ 51.2 yield (in nmol) InfA fw AAG ACC AAT CCT GTC ACC TCT GA 201.2 yield (in nmol) InfA Rw CAA AGC GTC TAC GCT GCA GTC C 201.2 yield (in nmol) InfB Fw GAG ACA CAA TTG CCT ACC TGC TT 201.2 yield (in nmol) InfB Rw TTC TTT CCC ACC GAA CCA AC 201.2 yield (in nmol) 2019-nCoV_N1-F GAC CCC AAA ATC AGC GAA AT 201.2 yield (in nmol) 2019-nCoV_N1-R TCT GGT TAC TGC CAG TTG AAT CTG 201.2 yield (in nmol) 2019-nCoV_N1-P /5TEX615/ACC CCG CATTAC GTT TGG TGG ACC/3IAbRQSp/ 51.2 yield (in nmol) RP-F AGA TTT GGA CCT GCG AGC G 201.2 yield (in nmol) RP-R GAG CGG CTG TCT CCA CAA GT 201.2 yield (in nmol) RP-P /5Cy5/TTC TGA CCT/TAO/ GAA GGC TCT GCG CG/3IAbRQSp/ 51.2 yield (in nmol)